Hotspot

ycf2-trnI-CAU

IRa · 163455-163782 · 328 bp

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Pi 0.0355
MarkerSeek score 63.30
Primer available yes
Label ycf2-trnI-CAU

Feature Metadata

label_name
ycf2-trnI-CAU
feature_type
igs
region
IRa
length_bp
328
variable_sites
17
pi
0.0355
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.89
markerseek_score
63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf2-trnI-CAU_p1 GTTGAACCCTGTGAATTGCG GTTGGGCGCTTTAACCATTC 50.0 50.0 57.9 58.0 509-644 bp 1.0 86.0
ycf2-trnI-CAU_p2 TCCAGGAAACAGCTCTCAGA GTTGGGCGCTTTAACCATTC 50.0 50.0 58.0 58.0 767-902 bp 1.0 85.7
ycf2-trnI-CAU_p3 ACTACACCCCTGATCGTGAA GTTGGGCGCTTTAACCATTC 50.0 50.0 58.1 58.0 542-677 bp 1.0 85.7
ycf2-trnI-CAU_p4 CCGGGTCCAATGGAATTCAT GTTGGGCGCTTTAACCATTC 50.0 50.0 57.9 58.0 659-794 bp 1.0 85.4
ycf2-trnI-CAU_p5 AATCATGGTCCTTGCGGATC GTTGGGCGCTTTAACCATTC 50.0 50.0 58.0 58.0 1000-1135 bp 1.0 85.3