Hotspot

rpl33-rps18

LSC · 74480-75655 · 1176 bp

Back to Durio

Pi 0.0166
MarkerSeek score 75.20
Primer available yes
Label rpl33-rps18

Feature Metadata

label_name
rpl33-rps18
feature_type
igs
region
LSC
length_bp
1176
variable_sites
44
pi
0.0166
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.95
markerseek_score
75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl33-rps18_p1 CGACACAATACGCCTAGTCG TCAATTCGATCCCCCGATTG 55.0 50.0 58.5 57.7 1331-1935 bp 1.0 80.0
rpl33-rps18_p2 GACACAATACGCCTAGTCGA TCAATTCGATCCCCCGATTG 50.0 50.0 57.2 57.7 1330-1934 bp 1.0 77.6
rpl33-rps18_p3 TCGACACAATACGCCTAGTC TCAATTCGATCCCCCGATTG 50.0 50.0 57.2 57.7 1332-1936 bp 1.0 77.6
rpl33-rps18_p4 CGACACAATACGCCTAGTCG ATTCGATCCCCCGATTGGAT 55.0 50.0 58.5 58.6 1328-1932 bp 1.0 77.0
rpl33-rps18_p5 ACGCCTAGTCGATTGGAATT TCAATTCGATCCCCCGATTG 45.0 50.0 57.0 57.7 1322-1926 bp 1.0 76.5