Hotspot

ndhC-trnV-UAC

LSC · 56137-57105 · 969 bp

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Pi 0.0133
MarkerSeek score 74.20
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
969
variable_sites
28
pi
0.0133
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
74.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 ACCCTTTCTAATCGGGGCTA AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.8 57.8 1113-1217 bp 1.0 82.3
ndhC-trnV-UAC_p2 CGGGGCTAAAACTCCAGAAA AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.8 57.8 1101-1205 bp 1.0 82.3
ndhC-trnV-UAC_p3 ATCGGGGCTAAAACTCCAGA AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.4 57.8 1103-1207 bp 1.0 80.5
ndhC-trnV-UAC_p4 AATCGGGGCTAAAACTCCAG AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.6 57.8 1104-1208 bp 1.0 80.5
ndhC-trnV-UAC_p5 ACCCTTTCTAATCGGGGCTA GAAGGTCTACGGTTCGAGTC 50.0 55.0 57.8 57.5 1112-1216 bp 1.0 79.6