Pi
0.0051
MarkerSeek score
64.90
Primer available
yes
Label
trnT-UGU-trnL-UAA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnT-UGU-trnL-UAA_p1 | GCGATGCTCTAACCTCTGAG | ACCAATTTCGCCATATCCCC | 55.0 | 50.0 | 57.9 | 58.0 | 1749-1859 bp | 1.0 | 81.7 |
| trnT-UGU-trnL-UAA_p2 | GCGATGCTCTAACCTCTGAG | TAGCGTCTACCAATTTCGCC | 55.0 | 50.0 | 57.9 | 58.1 | 1757-1867 bp | 1.0 | 81.1 |
| trnT-UGU-trnL-UAA_p3 | GGAATCGAACCGATGACCAT | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 57.8 | 58.0 | 1781-1891 bp | 1.0 | 80.8 |
| trnT-UGU-trnL-UAA_p4 | GCGATGCTCTAACCTCTGAG | CCAAGGCTCAATCCAATCCA | 55.0 | 50.0 | 57.9 | 57.9 | 1787-1897 bp | 1.0 | 80.5 |
| trnT-UGU-trnL-UAA_p5 | GCGATGCTCTAACCTCTGAG | ACCAAGGCTCAATCCAATCC | 55.0 | 50.0 | 57.9 | 57.9 | 1788-1898 bp | 1.0 | 80.4 |