Hotspot

atpF-atpH

LSC · 14486-15094 · 609 bp

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Pi 0.00346
MarkerSeek score 63.70
Primer available yes
Label atpF-atpH

Feature Metadata

label_name
atpF-atpH
feature_type
igs
region
LSC
length_bp
609
variable_sites
5
pi
0.00346
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpF-atpH_p1 TTAAATCCGAAACTCCCGGC TGGTTGTAGCATTGGCACTT 50.0 45.0 58.3 58.0 718-729 bp 1.0 79.9
atpF-atpH_p2 CAGTGACCCAAGGAAAGGAA TGGTTGTAGCATTGGCACTT 50.0 45.0 57.3 58.0 691-702 bp 1.0 76.8
atpF-atpH_p3 TTAAATCCGAAACTCCCGGC CTGGTTGTAGCATTGGCACT 50.0 50.0 58.3 58.5 719-730 bp 1.0 76.2
atpF-atpH_p4 TTAAATCCGAAACTCCCGGC ATGGGCTGGTTGTAGCATTG 50.0 50.0 58.3 58.5 724-735 bp 1.0 75.7
atpF-atpH_p5 TTAAATCCGAAACTCCCGGC CCGAGGCAGAGGGAAAAATA 50.0 50.0 58.3 57.3 793-804 bp 1.0 74.1