primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	20	20	55.0	55.0	58.1	57.9	0.0	0.0	0.203648	483	1293	878-987	1.000000	85.4
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	CAATCCACTGCCTTGATCCA	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.208010	483	1293	892-1001	1.000000	85.3
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	AATCCACTGCCTTGATCCAC	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.209518	483	1293	891-1000	1.000000	85.3
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	ACAATCCACTGCCTTGATCC	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.209518	483	1293	893-1002	1.000000	85.2
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	ATGTATATGGGCGAACGACG	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.135054	483	1293	940-1912	1.000000	84.5
atpF-atpH_p1	atpF-atpH	1	TTAAATCCGAAACTCCCGGC	TGGTTGTAGCATTGGCACTT	20	20	50.0	45.0	58.3	58.0	0.0	0.0	0.264516	14486	15094	718-729	1.000000	79.9
atpF-atpH_p2	atpF-atpH	2	CAGTGACCCAAGGAAAGGAA	TGGTTGTAGCATTGGCACTT	20	20	50.0	45.0	57.3	58.0	0.0	0.0	0.662991	14486	15094	691-702	1.000000	76.8
atpF-atpH_p3	atpF-atpH	3	TTAAATCCGAAACTCCCGGC	CTGGTTGTAGCATTGGCACT	20	20	50.0	50.0	58.3	58.5	0.0	0.0	0.720424	14486	15094	719-730	1.000000	76.2
atpF-atpH_p4	atpF-atpH	4	TTAAATCCGAAACTCCCGGC	ATGGGCTGGTTGTAGCATTG	20	20	50.0	50.0	58.3	58.5	0.0	0.0	0.780919	14486	15094	724-735	1.000000	75.7
atpF-atpH_p5	atpF-atpH	5	TTAAATCCGAAACTCCCGGC	CCGAGGCAGAGGGAAAAATA	20	20	50.0	50.0	58.3	57.3	0.0	0.0	0.968568	14486	15094	793-804	1.000000	74.1
psbZ-trnG-GCC_p1	psbZ-trnG-GCC	1	TTGCTTCTCCTGATGGTTGG	CGTCTTCTCCTTGGCAAAGA	20	20	50.0	50.0	57.8	57.8	0.0	1.1	0.374855	39135	40147	1114-1216	1.000000	83.4
psbZ-trnG-GCC_p2	psbZ-trnG-GCC	2	GCTTCTCCTGATGGTTGGTT	CGTCTTCTCCTTGGCAAAGA	20	20	50.0	50.0	57.8	57.8	0.0	1.1	0.376409	39135	40147	1112-1214	1.000000	83.3
psbZ-trnG-GCC_p3	psbZ-trnG-GCC	3	CTCCTGATGGTTGGTTGAGT	CGTCTTCTCCTTGGCAAAGA	20	20	50.0	50.0	57.1	57.8	0.0	1.1	1.036941	39135	40147	1108-1210	1.000000	78.1
psbZ-trnG-GCC_p4	psbZ-trnG-GCC	4	TGCTTCTCCTGATGGTTGGT	CGTCTTCTCCTTGGCAAAGA	20	20	50.0	50.0	58.9	57.8	0.0	1.1	1.102537	39135	40147	1113-1215	1.000000	77.5
psbZ-trnG-GCC_p5	psbZ-trnG-GCC	5	TCTCCTGATGGTTGGTTGAG	CGTCTTCTCCTTGGCAAAGA	20	20	50.0	50.0	56.8	57.8	0.0	1.1	1.328499	39135	40147	1109-1211	1.000000	75.7
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	50906	52604	1749-1859	1.000000	81.7
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	50906	52604	1757-1867	1.000000	81.1
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	15.5	0.0	0.245117	50906	52604	1781-1891	1.000000	80.8
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GCGATGCTCTAACCTCTGAG	CCAAGGCTCAATCCAATCCA	20	20	55.0	50.0	57.9	57.9	0.0	0.0	0.281186	50906	52604	1787-1897	1.000000	80.5
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GCGATGCTCTAACCTCTGAG	ACCAAGGCTCAATCCAATCC	20	20	55.0	50.0	57.9	57.9	0.0	0.0	0.282713	50906	52604	1788-1898	1.000000	80.4
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	ACCCTTTCTAATCGGGGCTA	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	57.8	57.8	0.0	0.0	0.382946	56137	57105	1113-1217	1.000000	82.3
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	CGGGGCTAAAACTCCAGAAA	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	57.8	57.8	0.0	0.0	0.391484	56137	57105	1101-1205	1.000000	82.3
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	ATCGGGGCTAAAACTCCAGA	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	58.4	57.8	0.0	0.0	0.617224	56137	57105	1103-1207	1.000000	80.5
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	AATCGGGGCTAAAACTCCAG	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	57.6	57.8	0.0	0.0	0.617342	56137	57105	1104-1208	1.000000	80.5
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	ACCCTTTCTAATCGGGGCTA	GAAGGTCTACGGTTCGAGTC	20	20	50.0	55.0	57.8	57.5	0.0	0.0	0.726718	56137	57105	1112-1216	1.000000	79.6
rpl33-rps18_p1	rpl33-rps18	1	CGACACAATACGCCTAGTCG	TCAATTCGATCCCCCGATTG	20	20	55.0	50.0	58.5	57.7	0.0	0.0	0.790741	74480	75655	1331-1935	1.000000	80.0
rpl33-rps18_p2	rpl33-rps18	2	GACACAATACGCCTAGTCGA	TCAATTCGATCCCCCGATTG	20	20	50.0	50.0	57.2	57.7	0.0	0.0	1.092932	74480	75655	1330-1934	1.000000	77.6
rpl33-rps18_p3	rpl33-rps18	3	TCGACACAATACGCCTAGTC	TCAATTCGATCCCCCGATTG	20	20	50.0	50.0	57.2	57.7	0.0	0.0	1.092932	74480	75655	1332-1936	1.000000	77.6
rpl33-rps18_p4	rpl33-rps18	4	CGACACAATACGCCTAGTCG	ATTCGATCCCCCGATTGGAT	20	20	55.0	50.0	58.5	58.6	0.0	0.0	1.173970	74480	75655	1328-1932	1.000000	77.0
rpl33-rps18_p5	rpl33-rps18	5	ACGCCTAGTCGATTGGAATT	TCAATTCGATCCCCCGATTG	20	20	45.0	50.0	57.0	57.7	0.0	0.0	1.236405	74480	75655	1322-1926	1.000000	76.5
clpP1_p1	clpP1	1	TGACCCTGTTAGTCCGTTCT	AAGCAACGGAGCCATCATAG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.046329	78154	78273	1352-1841	1.000000	88.6
clpP1_p2	clpP1	2	TGACCCTGTTAGTCCGTTCT	CTCGAATCAACTTGCGGGTA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.009953	78154	78273	1993-2479	1.000000	88.1
clpP1_p3	clpP1	3	TGACCCTGTTAGTCCGTTCT	CACTAGCCATGGGATTGACC	20	20	50.0	55.0	58.0	58.0	0.0	15.1	0.032498	78154	78273	1844-2330	1.000000	88.1
clpP1_p4	clpP1	4	CCTCTATACACCGGAGCTCA	CTCGAATCAACTTGCGGGTA	20	20	55.0	50.0	58.0	58.0	17.2	0.0	0.021922	78154	78273	2155-2641	1.000000	87.7
clpP1_p5	clpP1	5	CCTCTATACACCGGAGCTCA	CACTAGCCATGGGATTGACC	20	20	55.0	55.0	58.0	58.0	17.2	15.1	0.044468	78154	78273	2006-2492	1.000000	87.7
rpl16_p1	rpl16	1	AAAAGGAAACCCGCAATTGT	TGAGCAGTTCTAAATTCTATAAGGT	20	25	40.0	32.0	56.7	55.8	7.4	0.0	8.487914	90029	91690	1728-1785	0.800000	35.8
rpl16_p2	rpl16	2	AAAGGAAACCCGCAATTGTT	TGAGCAGTTCTAAATTCTATAAGGT	20	25	40.0	32.0	56.7	55.8	7.4	0.0	8.487914	90029	91690	1727-1784	0.800000	35.8
rpl16_p3	rpl16	3	AAGGAAACCCGCAATTGTTT	TGAGCAGTTCTAAATTCTATAAGGT	20	25	40.0	32.0	56.7	55.8	7.4	0.0	8.487914	90029	91690	1726-1783	0.800000	35.8
rpl16_p4	rpl16	4	AGGAAACCCGCAATTGTTTT	TGAGCAGTTCTAAATTCTATAAGGT	20	25	40.0	32.0	56.7	55.8	7.4	0.0	8.487914	90029	91690	1725-1782	0.800000	35.8
rpl16_p5	rpl16	5	AGGAAACCCGCAATTGTTTTT	TGAGCAGTTCTAAATTCTATAAGGT	21	25	38.1	32.0	57.4	55.8	7.4	0.0	8.749120	90029	91690	1725-1782	0.800000	35.8
trnI-CAU-ycf2_p1	trnI-CAU-ycf2	1	GTTGGGCGCTTTAACCATTC	GTTGAACCCTGTGAATTGCG	20	20	50.0	50.0	58.0	57.9	7.6	0.0	0.059678	95636	95963	509-644	1.000000	86.5
trnI-CAU-ycf2_p2	trnI-CAU-ycf2	2	GTTGGGCGCTTTAACCATTC	ACTACACCCCTGATCGTGAA	20	20	50.0	50.0	58.0	58.1	7.6	5.6	0.076179	95636	95963	542-677	1.000000	86.2
trnI-CAU-ycf2_p3	trnI-CAU-ycf2	3	GTTGGGCGCTTTAACCATTC	TCCAGGAAACAGCTCTCAGA	20	20	50.0	50.0	58.0	58.0	7.6	0.0	0.024657	95636	95963	767-902	1.000000	86.1
trnI-CAU-ycf2_p4	trnI-CAU-ycf2	4	GTTGGGCGCTTTAACCATTC	CCGGGTCCAATGGAATTCAT	20	20	50.0	50.0	58.0	57.9	7.6	2.3	0.080978	95636	95963	659-794	1.000000	85.9
trnI-CAU-ycf2_p5	trnI-CAU-ycf2	5	GTTGGGCGCTTTAACCATTC	AATCATGGTCCTTGCGGATC	20	20	50.0	50.0	58.0	58.0	7.6	0.0	0.036329	95636	95963	1000-1135	1.000000	85.6
trnN-GUU-ndhF_p1	trnN-GUU-ndhF	1	GGGTTACCTGCTCTTAACGG	ATATGTAGGGGGAAGTCGCA	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.117058	118910	119485	792-841	0.800000	80.1
trnN-GUU-ndhF_p2	trnN-GUU-ndhF	2	GGGTTACCTGCTCTTAACGG	TATGTAGGGGGAAGTCGCAT	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.117058	118910	119485	791-840	0.800000	80.1
trnN-GUU-ndhF_p3	trnN-GUU-ndhF	3	GGGTTACCTGCTCTTAACGG	GGGGAAGTCGCATCTCTTTT	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.135290	118910	119485	784-833	0.800000	80.0
trnN-GUU-ndhF_p4	trnN-GUU-ndhF	4	GGCAGAATAGCAGAGCAAGT	ATATGTAGGGGGAAGTCGCA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.132640	118910	119485	1089-1138	0.800000	78.6
trnN-GUU-ndhF_p5	trnN-GUU-ndhF	5	GGCAGAATAGCAGAGCAAGT	TATGTAGGGGGAAGTCGCAT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.132640	118910	119485	1088-1137	0.800000	78.6
ndhF_p1	ndhF	1	GAGCTACTGAGGAACAACGG	AGAGCCGGTGAATCAGAAAC	20	20	55.0	50.0	58.0	57.9	0.0	1.9	0.102936	119486	121735	2724-2892	1.000000	85.5
ndhF_p2	ndhF	2	CCCGGAACTTCTTCGTAGTG	AGAGCCGGTGAATCAGAAAC	20	20	55.0	50.0	58.0	57.9	0.7	1.9	0.108435	119486	121735	2628-2797	1.000000	85.5
ndhF_p3	ndhF	3	CCGGAACTTCTTCGTAGTGG	AGAGCCGGTGAATCAGAAAC	20	20	55.0	50.0	58.0	57.9	0.0	1.9	0.108435	119486	121735	2627-2796	1.000000	85.5
ndhF_p4	ndhF	4	CAATTCCCGTTCTCAACCCA	AGAGCCGGTGAATCAGAAAC	20	20	50.0	50.0	58.1	57.9	0.0	1.9	0.196318	119486	121735	2683-2851	1.000000	84.8
ndhF_p5	ndhF	5	GTAACCCCCGGAACTTCTTC	AGAGCCGGTGAATCAGAAAC	20	20	55.0	50.0	57.9	57.9	0.7	1.9	0.205611	119486	121735	2634-2803	1.000000	84.7
ndhF-rpl32_p1	ndhF-rpl32	1	TGTTGCCGTCGGAAAAAGTA	TGAAAAAGCTTTCAACGCCG	20	20	45.0	45.0	58.0	58.2	17.5	13.1	0.215312	121736	122637	1080-1101	1.000000	80.5
ndhF-rpl32_p2	ndhF-rpl32	2	TTGGAAGTGGCACAAAAGGT	TGAAAAAGCTTTCAACGCCG	20	20	45.0	45.0	58.1	58.2	21.4	13.1	0.306838	121736	122637	1033-1054	1.000000	79.9
ndhF-rpl32_p3	ndhF-rpl32	3	TGTTGCCGTCGGAAAAAGTA	GCCGCCCAATATCCTTTCTT	20	20	45.0	50.0	58.0	58.3	17.5	0.0	0.356761	121736	122637	1064-1085	1.000000	79.4
ndhF-rpl32_p4	ndhF-rpl32	4	TTTGTTGCCGTCGGAAAAAG	TGAAAAAGCTTTCAACGCCG	20	20	45.0	45.0	57.8	58.2	17.5	13.1	0.362435	121736	122637	1082-1103	1.000000	79.4
ndhF-rpl32_p5	ndhF-rpl32	5	TTGGAAGTGGCACAAAAGGT	GCCGCCCAATATCCTTTCTT	20	20	45.0	50.0	58.1	58.3	21.4	0.0	0.448287	121736	122637	1017-1038	1.000000	78.8
ycf1_p1	ycf1	1	GCCCGGATCAAAAGATTCCT	AGAAAACCGTCACACATGCT	20	20	50.0	45.0	57.9	58.0	0.7	0.0	0.091274	138736	138855	666-876	1.000000	88.2
ycf1_p2	ycf1	2	GCCCGGATCAAAAGATTCCT	GAAAACCGTCACACATGCTG	20	20	50.0	50.0	57.9	58.0	0.7	0.0	0.095149	138736	138855	665-875	1.000000	88.2
ycf1_p3	ycf1	3	GCCCGGATCAAAAGATTCCT	CCGTCACACATGCTGACTAA	20	20	50.0	50.0	57.9	57.9	0.7	17.6	0.137441	138736	138855	660-870	1.000000	87.9
ycf1_p4	ycf1	4	GCCCGGATCAAAAGATTCCT	AGCAACAACCGGCTTTCTTA	20	20	50.0	45.0	57.9	58.0	0.7	0.0	0.082933	138736	138855	1258-1468	1.000000	84.7
ycf1_p5	ycf1	5	GCCCGGATCAAAAGATTCCT	GACTGCCGGAAATTCAATGC	20	20	50.0	50.0	57.9	58.1	0.7	5.0	0.145276	138736	138855	1075-1111	0.800000	76.6
ycf1-trnN-GUU_p1	ycf1-trnN-GUU	1	TAAGAAAGCCGGTTGTTGCT	GGGTTACCTGCTCTTAACGG	20	20	45.0	55.0	58.0	58.0	1.9	0.0	0.044288	139768	140508	1103-1294	1.000000	91.6
ycf1-trnN-GUU_p2	ycf1-trnN-GUU	2	TAAGAAAGCCGGTTGTTGCT	TCGGGAGAATCGATGACTGA	20	20	45.0	50.0	58.0	57.9	1.9	0.0	0.077123	139768	140508	1312-1503	1.000000	90.2
ycf1-trnN-GUU_p3	ycf1-trnN-GUU	3	TAAGAAAGCCGGTTGTTGCT	GGCAGAATAGCAGAGCAAGT	20	20	45.0	50.0	58.0	58.0	1.9	0.0	0.059870	139768	140508	1400-1591	1.000000	90.0
ycf1-trnN-GUU_p4	ycf1-trnN-GUU	4	TAAGAAAGCCGGTTGTTGCT	TAATGTGTTTGCTCGCGGTA	20	20	45.0	45.0	58.0	57.9	1.9	6.1	0.106066	139768	140508	1343-1534	1.000000	89.8
ycf1-trnN-GUU_p5	ycf1-trnN-GUU	5	GCATTGAATTTCCGGCAGTC	GGGTTACCTGCTCTTAACGG	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.106631	139768	140508	1288-1477	0.800000	72.0
ycf2-trnI-CAU_p1	ycf2-trnI-CAU	1	GTTGAACCCTGTGAATTGCG	GTTGGGCGCTTTAACCATTC	20	20	50.0	50.0	57.9	58.0	0.0	7.6	0.059678	163455	163782	509-644	1.000000	86.0
ycf2-trnI-CAU_p2	ycf2-trnI-CAU	2	TCCAGGAAACAGCTCTCAGA	GTTGGGCGCTTTAACCATTC	20	20	50.0	50.0	58.0	58.0	0.0	7.6	0.024657	163455	163782	767-902	1.000000	85.7
ycf2-trnI-CAU_p3	ycf2-trnI-CAU	3	ACTACACCCCTGATCGTGAA	GTTGGGCGCTTTAACCATTC	20	20	50.0	50.0	58.1	58.0	5.6	7.6	0.076179	163455	163782	542-677	1.000000	85.7
ycf2-trnI-CAU_p4	ycf2-trnI-CAU	4	CCGGGTCCAATGGAATTCAT	GTTGGGCGCTTTAACCATTC	20	20	50.0	50.0	57.9	58.0	2.3	7.6	0.080978	163455	163782	659-794	1.000000	85.4
ycf2-trnI-CAU_p5	ycf2-trnI-CAU	5	AATCATGGTCCTTGCGGATC	GTTGGGCGCTTTAACCATTC	20	20	50.0	50.0	58.0	58.0	0.0	7.6	0.036329	163455	163782	1000-1135	1.000000	85.3