Hotspot

ycf1

IRb · 111653-112774 · 1122 bp

Back to Dunalia

Pi 0.00096

MarkerSeek score 48.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: IRb
length_bp: 1122
variable_sites: 1
pi: 0.00096
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 48.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CCCGGAACTTCTTCGTAGTG	TCAACCAAGAGGGAGGGAAT	55.0	50.0	58.0	57.9	1841-1915 bp	1.0	83.0
ycf1_p2	CCGGAACTTCTTCGTAGTGG	TCAACCAAGAGGGAGGGAAT	55.0	50.0	58.0	57.9	1840-1914 bp	1.0	83.0
ycf1_p3	GCGAGCCCCTTTATTCTGAA	TCAACCAAGAGGGAGGGAAT	50.0	50.0	58.0	57.9	2040-2114 bp	1.0	82.5
ycf1_p4	AAGCGAGCCCCTTTATTCTG	TCAACCAAGAGGGAGGGAAT	50.0	50.0	58.0	57.9	2042-2116 bp	1.0	82.4
ycf1_p5	CAATTGTGACCTCTCGGGAG	TCAACCAAGAGGGAGGGAAT	55.0	50.0	58.0	57.9	2279-2353 bp	1.0	82.3