Hotspot

rps15-ycf1

SSC · 126122-126517 · 396 bp

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Pi 0.00505

MarkerSeek score 55.80

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 396
variable_sites: 2
pi: 0.00505
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	AGTCTCCGTATCTTATTGGTGA	TGGTAGCCGTTTCAGTATCT	40.9	45.0	56.1	56.0	552 bp	1.0	40.3
rps15-ycf1_p2	TGTGAAGTAAGTCTCCGTATCT	TGGTAGCCGTTTCAGTATCT	40.9	45.0	56.1	56.0	561 bp	1.0	40.3
rps15-ycf1_p3	AATGTGAAGTAAGTCTCCGT	TGGTAGCCGTTTCAGTATCT	40.0	45.0	54.0	56.0	563 bp	1.0	40.3
rps15-ycf1_p4	AAATGTGAAGTAAGTCTCCGT	TGGTAGCCGTTTCAGTATCT	38.1	45.0	54.9	56.0	564 bp	1.0	40.3
rps15-ycf1_p5	AGTCTCCGTATCTTATTGGTGA	TGGTAGCCGTTTCAGTATCTT	40.9	42.9	56.1	56.7	552 bp	1.0	40.3