Hotspot

rpl32-trnL-UAG

SSC · 115955-116779 · 825 bp

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Pi 0.00365

MarkerSeek score 61.10

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 825
variable_sites: 3
pi: 0.00365
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AGGAAGGGATATTGGGTAGCA	TCCTAAGAGCAGCGTGTCTA	47.6	50.0	57.9	57.9	968-969 bp	1.0	70.8
rpl32-trnL-UAG_p2	AGGAAGGGATATTGGGTAGCA	TCGAACCGAGATGCATTAGC	47.6	50.0	57.9	58.1	994-995 bp	1.0	70.6
rpl32-trnL-UAG_p3	AGGAAGGGATATTGGGTAGCA	AGCAGCGTGTCTACCAATTT	47.6	45.0	57.9	57.8	961-962 bp	1.0	70.3
rpl32-trnL-UAG_p4	AGGAAGGGATATTGGGTAGCA	CCTAAGAGCAGCGTGTCTAC	47.6	55.0	57.9	57.8	967-968 bp	1.0	70.2
rpl32-trnL-UAG_p5	AGGAAGGGATATTGGGTAGCA	CTAAGAGCAGCGTGTCTACC	47.6	55.0	57.9	57.8	966-967 bp	1.0	70.2