Hotspot

ycf1-ndhF

SSC · 112775-112785 · 11 bp

Back to Dunalia

Pi 0.273

MarkerSeek score 75.60

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: SSC
length_bp: 11
variable_sites: 3
pi: 0.273
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	TTGCAACTGCTTGATAAAAA	GCGGACGCATCTCTTCTTAT	30.0	50.0	52.1	57.9	139-145 bp	1.0	41.7
ycf1-ndhF_p2	AAATTGCAACTGCTTGATAAA	GCGGACGCATCTCTTCTTAT	28.6	50.0	52.8	57.9	142-148 bp	1.0	41.7
ycf1-ndhF_p3	AATTGCAACTGCTTGATAAAA	GCGGACGCATCTCTTCTTAT	28.6	50.0	52.8	57.9	141-147 bp	1.0	41.7
ycf1-ndhF_p4	ATTGCAACTGCTTGATAAAAA	GCGGACGCATCTCTTCTTAT	28.6	50.0	52.8	57.9	140-146 bp	1.0	41.7
ycf1-ndhF_p5	AATTGCAACTGCTTGATAAAAA	GCGGACGCATCTCTTCTTAT	27.3	50.0	53.7	57.9	141-147 bp	1.0	41.7