Hotspot

petA-psbJ

LSC · 66067-66776 · 710 bp

Back to Dunalia

Pi 0.00141

MarkerSeek score 59.80

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 710
variable_sites: 1
pi: 0.00141
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GAGAAGGTTCAATTGGCCGA	GGTTCATATTCCGGGTTGGG	50.0	55.0	58.2	58.3	776-1130 bp	1.0	76.3
petA-psbJ_p2	TCGAGAAGGTTCAATTGGCC	GGTTCATATTCCGGGTTGGG	50.0	55.0	58.2	58.3	778-1132 bp	1.0	76.3
petA-psbJ_p3	AGAAGGTTCAATTGGCCGAA	GGTTCATATTCCGGGTTGGG	45.0	55.0	57.7	58.3	775-1129 bp	1.0	75.5
petA-psbJ_p4	GAGAAGGTTCAATTGGCCGA	CATATTCCGGGTTGGGTTCA	50.0	50.0	58.2	57.6	772-1126 bp	1.0	75.4
petA-psbJ_p5	GAGAAGGTTCAATTGGCCGA	TGGTTCATATTCCGGGTTGG	50.0	50.0	58.2	57.6	777-1131 bp	1.0	75.4