Hotspot

atpH-atpI

LSC · 14043-15265 · 1223 bp

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Pi 0.000822

MarkerSeek score 59.80

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1223
variable_sites: 1
pi: 0.000822
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TACCTGTCATGCTCCTTGGA	45.0	50.0	58.0	58.0	1352-1359 bp	1.0	79.8
atpH-atpI_p2	TACCCTCTACAGCTTGACCC	TACCTGTCATGCTCCTTGGA	55.0	50.0	58.1	58.0	1435-1442 bp	1.0	79.0
atpH-atpI_p3	GCGATACCCTCTACAGCTTG	TACCTGTCATGCTCCTTGGA	55.0	50.0	57.9	58.0	1439-1446 bp	1.0	78.9
atpH-atpI_p4	AATAACGGAAGCGGCAGAAA	TACCTGTCATGCTCCTTGGA	45.0	50.0	58.2	58.0	1353-1360 bp	1.0	78.6
atpH-atpI_p5	GCCAATCCAGCAGCAATAAC	TACCTGTCATGCTCCTTGGA	50.0	50.0	57.8	58.0	1367-1374 bp	1.0	78.4