Hotspot

ycf1

SSC · 121327-126423 · 5097 bp

Back to Duhaldea

Pi 0.0104
MarkerSeek score 49.70
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5097
variable_sites
53
pi
0.0104
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
49.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 TCCACGATACATCCCATTCA AGAATTGAGGCTTTAGAGACAGT 45.0 39.1 56.0 57.2 186 bp 1.0 43.0
ycf1_p2 CTTCCACGATACATCCCATTCA AGAATTGAGGCTTTAGAGACAGT 45.5 39.1 57.9 57.2 188 bp 1.0 43.0
ycf1_p3 TCCACGATACATCCCATTCAA AGAATTGAGGCTTTAGAGACAGT 42.9 39.1 56.8 57.2 186 bp 1.0 43.0
ycf1_p4 TTCTTCCACGATACATCCCA AGAATTGAGGCTTTAGAGACAGT 45.0 39.1 55.9 57.2 190 bp 1.0 42.9
ycf1_p5 CATTCTTCCACGATACATCCCA AGAATTGAGGCTTTAGAGACAGT 45.5 39.1 57.9 57.2 192 bp 1.0 42.9