Hotspot

ndhF

SSC · 107716-109944 · 2229 bp

Back to Duhaldea

Pi 0.0045

MarkerSeek score 45.50

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2229
variable_sites: 10
pi: 0.0045
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	CTAGCACTGGGTAGACCTCA	GAGGTAAGATCCGGTGAATCG	55.0	52.4	57.9	58.0	2606-2619 bp	1.0	71.8
ndhF_p2	TCGTTGTGGTCGGACTCTAT	GAGGTAAGATCCGGTGAATCG	50.0	52.4	58.2	58.0	2794-2807 bp	1.0	71.5
ndhF_p3	ATCAATTCGGTCGTTGTGGT	GAGGTAAGATCCGGTGAATCG	45.0	52.4	57.8	58.0	2804-2817 bp	1.0	71.4
ndhF_p4	TCTCTTCCTTCTCCGAGCTC	GAGGTAAGATCCGGTGAATCG	55.0	52.4	58.2	58.0	2736-2749 bp	1.0	70.9
ndhF_p5	CTAGCACTGGGTAGACCTCA	AGGTAAGATCCGGTGAATCG	55.0	50.0	57.9	56.8	2605-2618 bp	1.0	70.4