Hotspot

rpl32-trnL-UAG

SSC · 110833-111724 · 892 bp

Back to Duhaldea

Pi 0.0154
MarkerSeek score 64.00
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
892
variable_sites
11
pi
0.0154
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.80
markerseek_score
64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AAGGAAAGGATATTGGGCGG AGCGTGTCTACCGATTTCAC 50.0 50.0 57.6 58.0 857-1025 bp 1.0 82.0
rpl32-trnL-UAG_p2 AAGGAAAGGATATTGGGCGG CAGCGTGTCTACCGATTTCA 50.0 50.0 57.6 58.0 858-1026 bp 1.0 81.9
rpl32-trnL-UAG_p3 CGGCGTTAAAAGCTTTGTCA AGCGTGTCTACCGATTTCAC 45.0 50.0 57.6 58.0 840-1008 bp 1.0 81.7
rpl32-trnL-UAG_p4 CGGCGTTAAAAGCTTTGTCA CAGCGTGTCTACCGATTTCA 45.0 50.0 57.6 58.0 841-1009 bp 1.0 81.7
rpl32-trnL-UAG_p5 AAGGAAAGGATATTGGGCGG TCCTAAGAGCAGCGTGTCTA 50.0 50.0 57.6 57.9 867-1035 bp 1.0 81.0