Hotspot

psbM-trnD-GUC

LSC · 10892-11550 · 659 bp

Back to Duhaldea

Pi 0.0139

MarkerSeek score 65.30

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 659
variable_sites: 9
pi: 0.0139
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGTAGAAAGGCAGTAGGAACT	TTCAATAGGCAAGAGCACCG	42.9	50.0	55.7	58.3	781-791 bp	1.0	53.7
psbM-trnD-GUC_p2	GGAACTAAAATGAACAGTGCAGT	TTCAATAGGCAAGAGCACCG	39.1	50.0	57.6	58.3	766-776 bp	1.0	52.6
psbM-trnD-GUC_p3	AAAATGAACAGTGCAGTAGC	TTCAATAGGCAAGAGCACCG	40.0	50.0	54.5	58.3	760-770 bp	1.0	52.1
psbM-trnD-GUC_p4	AAGTAGAAAGGCAGTAGGAACT	TTCAATAGGCAAGAGCACCG	40.9	50.0	56.5	58.3	782-792 bp	1.0	51.8
psbM-trnD-GUC_p5	ACTAAAATGAACAGTGCAGTAGC	TTCAATAGGCAAGAGCACCG	39.1	50.0	57.4	58.3	763-773 bp	1.0	51.2