Hotspot

psbA-trnK-UUU

LSC · 1534-1755 · 222 bp

Back to Duhaldea

Pi 0.0327

MarkerSeek score 74.70

Primer available yes

Label psbA-trnK-UUU

Feature Metadata

label_name: psbA-trnK-UUU
feature_type: igs
region: LSC
length_bp: 222
variable_sites: 7
pi: 0.0327
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 74.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	TAAAGACGGTTTTCGGTGCT	GAAAGCCGTGTGCAATGAAA	45.0	45.0	57.8	57.9	423-432 bp	1.0	79.8
psbA-trnK-UUU_p2	TAAAGACGGTTTTCGGTGCT	CTAGTTCCGGGTTCGAATCC	45.0	55.0	57.8	57.8	338-347 bp	1.0	79.8
psbA-trnK-UUU_p3	GTAAAGACGGTTTTCGGTGC	GAAAGCCGTGTGCAATGAAA	50.0	45.0	57.7	57.9	424-433 bp	1.0	79.3
psbA-trnK-UUU_p4	GTAAAGACGGTTTTCGGTGC	CTAGTTCCGGGTTCGAATCC	50.0	55.0	57.7	57.8	339-348 bp	1.0	79.3
psbA-trnK-UUU_p5	TTTTCGGTGCTGGTTATCCA	GAAAGCCGTGTGCAATGAAA	45.0	45.0	57.4	57.9	414-423 bp	1.0	77.2