Hotspot

psaB

LSC · 37338-39542 · 2205 bp

Back to Dudleya

Pi 0.000907

MarkerSeek score 57.90

Primer available yes

Label psaB

Feature Metadata

label_name: psaB
feature_type: gene
region: LSC
length_bp: 2205
variable_sites: 3
pi: 0.000907
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaB_p1	TAGGACCCTTCTGCATCGAA	TACAAGGACGTGCTGTAGGA	50.0	50.0	58.1	58.1	2373 bp	1.0	78.3
psaB_p2	TAGGACCCTTCTGCATCGAA	AAGGACGTGCTGTAGGAGTA	50.0	50.0	58.1	57.8	2370 bp	1.0	77.3
psaB_p3	TAGGACCCTTCTGCATCGAA	AGGACGTGCTGTAGGAGTAA	50.0	50.0	58.1	57.8	2369 bp	1.0	77.3
psaB_p4	AGAACTAGGACCCTTCTGCA	TACAAGGACGTGCTGTAGGA	50.0	50.0	57.7	58.1	2378 bp	1.0	76.8
psaB_p5	TAGGACCCTTCTGCATCGAA	GACGTGCTGTAGGAGTAACC	50.0	55.0	58.1	57.7	2367 bp	1.0	76.8