Hotspot

clpP1

LSC · 68058-70072 · 2015 bp

Back to Dudleya

Pi 0.00132

MarkerSeek score 57.90

Primer available yes

Label clpP1

Feature Metadata

label_name: clpP1
feature_type: gene
region: LSC
length_bp: 2015
variable_sites: 4
pi: 0.00132
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP1_p1	AATCGCGGTTGTGAAATCCT	ACCCACTATTACGTTTTCACA	45.0	38.1	58.2	55.2	2102-2108 bp	1.0	48.1
clpP1_p2	ATCGCGGTTGTGAAATCCTT	ACCCACTATTACGTTTTCACA	45.0	38.1	58.2	55.2	2101-2107 bp	1.0	48.1
clpP1_p3	CGCTCCTAACCGAATGCTTA	ACCCACTATTACGTTTTCACA	50.0	38.1	57.8	55.2	2186-2192 bp	1.0	47.7
clpP1_p4	AATCGCGGTTGTGAAATCCT	AACCCACTATTACGTTTTCACA	45.0	36.4	58.2	55.9	2103-2109 bp	1.0	46.3
clpP1_p5	ATCGCGGTTGTGAAATCCTT	AACCCACTATTACGTTTTCACA	45.0	36.4	58.2	55.9	2102-2108 bp	1.0	46.3