Hotspot

atpF

LSC · 12010-13295 · 1286 bp

Back to Dudleya

Pi 0.00156

MarkerSeek score 58.30

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1286
variable_sites: 3
pi: 0.00156
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	CGCTCAATACGGTCACGAAT	GGGCGAAGTGATCCAAAAAG	50.0	50.0	58.4	57.4	1455-1456 bp	1.0	71.5
atpF_p2	CGCTCAATACGGTCACGAAT	GGGGCGAAGTGATCCAAAAA	50.0	50.0	58.4	58.7	1456-1457 bp	1.0	70.6
atpF_p3	CGCTCAATACGGTCACGAAT	GGGGCGAAGTGATCCAAAA	50.0	52.6	58.4	58.0	1456-1457 bp	1.0	68.3
atpF_p4	CGCTCAATACGGTCACGAA	GGGCGAAGTGATCCAAAAAG	52.6	50.0	58.0	57.4	1455-1456 bp	1.0	66.9
atpF_p5	CGCTCAATACGGTCACGAA	GGGGCGAAGTGATCCAAAAA	52.6	50.0	58.0	58.7	1456-1457 bp	1.0	66.0