Hotspot

petA-psbJ

LSC · 61571-62298 · 728 bp

Back to Dudleya

Pi 0.00549

MarkerSeek score 45.90

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 728
variable_sites: 6
pi: 0.00549
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCCTTTTGTTCTTCTTGGC	ATTCGGGATTGGGTTCATCC	50.0	50.0	57.8	57.6	856-857 bp	1.0	76.4
petA-psbJ_p2	CCATTACGTGTTCAAGGCCT	ATTCGGGATTGGGTTCATCC	50.0	50.0	58.2	57.6	871-872 bp	1.0	76.2
petA-psbJ_p3	TCCATTACGTGTTCAAGGCC	ATTCGGGATTGGGTTCATCC	50.0	50.0	58.2	57.6	872-873 bp	1.0	76.2
petA-psbJ_p4	GGCCTTTTGTTCTTCTTGGC	GGGATTGGGTTCATCCTTGT	50.0	50.0	57.8	57.5	852-853 bp	1.0	75.1
petA-psbJ_p5	CCATTACGTGTTCAAGGCCT	GGGATTGGGTTCATCCTTGT	50.0	50.0	58.2	57.5	867-868 bp	1.0	74.9