Hotspot

psbC-trnS-UGA

LSC · 35042-35219 · 178 bp

Back to Dudleya

Pi 0.00936

MarkerSeek score 58.80

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 178
variable_sites: 2
pi: 0.00936
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	CCTGTTCTTTCCATGACCCC	TCTTTCGGAGAGATGGCTGA	55.0	50.0	58.2	58.1	306-307 bp	1.0	78.3
psbC-trnS-UGA_p2	CCTGTTCTTTCCATGACCCC	GGGTTCGAATCCCTCTCTCT	55.0	55.0	58.2	58.2	230-231 bp	1.0	78.0
psbC-trnS-UGA_p3	AGCCTGTTCTTTCCATGACC	TCTTTCGGAGAGATGGCTGA	50.0	50.0	57.8	58.1	308-309 bp	1.0	77.9
psbC-trnS-UGA_p4	CCTGTTCTTTCCATGACCCC	CTTTCGGAGAGATGGCTGAG	55.0	55.0	58.2	57.8	305-306 bp	1.0	77.7
psbC-trnS-UGA_p5	AGCCTGTTCTTTCCATGACC	GGGTTCGAATCCCTCTCTCT	50.0	55.0	57.8	58.2	232-233 bp	1.0	77.6