Hotspot

trnT-GGU-psbD

LSC · 31877-32610 · 734 bp

Back to Dudleya

Pi 0.00537

MarkerSeek score 65.70

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 734
variable_sites: 4
pi: 0.00537
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	55.0	45.0	58.1	58.2	828-884 bp	1.0	81.9
trnT-GGU-psbD_p2	GTGGTAGAGTAACGCCATGG	GACCGGACCAACCTACAAAA	55.0	50.0	58.1	57.7	842-898 bp	1.0	81.8
trnT-GGU-psbD_p3	TCAGTGGTAGAGTAACGCCA	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.1	58.2	831-887 bp	1.0	81.7
trnT-GGU-psbD_p4	TCAGTGGTAGAGTAACGCCA	GACCGGACCAACCTACAAAA	50.0	50.0	58.1	57.7	845-901 bp	1.0	81.6
trnT-GGU-psbD_p5	GTGGTAGAGTAACGCCATGG	TAGACCGGACCAACCTACAA	55.0	50.0	58.1	57.7	844-900 bp	1.0	81.4