Hotspot

accD

Genome · 34378-35469 · 1092 bp

Back to Drypetes

Pi 0.0838

MarkerSeek score 83.10

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: Genome
length_bp: 1092
variable_sites: 189
pi: 0.0838
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 83.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	TTACGCCTGCATAAGAACCC	CAAAAGGAAACGTCGAACCG	50.0	50.0	58.0	58.0	2090-2296 bp	0.666667	78.3
accD_p2	CCATTGCAATTGCCGGAAAT	CAAAAGGAAACGTCGAACCG	45.0	50.0	58.0	58.0	1913-2168 bp	0.666667	77.8
accD_p3	CCATTGCAATTGCCGGAAAT	TCGAGTTTGATCTGCTGAGC	45.0	50.0	58.0	58.0	1684 bp	0.166667	46.4
accD_p4	TTACGCCTGCATAAGAACCC	TCGAGTTTGATCTGCTGAGC	50.0	50.0	58.0	58.0	1861 bp	0.166667	46.4
accD_p5	TTGCCGGAAATACTAAGCCC	TCGAGTTTGATCTGCTGAGC	50.0	50.0	58.0	58.0	1675 bp	0.166667	46.3