Hotspot

trnQ-UUG-ndhF

Genome · 90363-90461 · 99 bp

Back to Drypetes

Pi 0.0458

MarkerSeek score 76.30

Primer available yes

Label trnQ-UUG-ndhF

Feature Metadata

label_name: trnQ-UUG-ndhF
feature_type: igs
region: Genome
length_bp: 99
variable_sites: 11
pi: 0.0458
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-ndhF_p1	TGGGCGAGACTTGACAAAAA	TGGGATTGCCAATGTAGTCG	45.0	50.0	58.0	58.0	389-833 bp	1.0	91.3
trnQ-UUG-ndhF_p2	TGGGCGAGACTTGACAAAAA	AAAATATGTGGGAGGCGGTC	45.0	50.0	58.0	57.9	338-782 bp	1.0	91.2
trnQ-UUG-ndhF_p3	CATAGTGGGCGAGACTTGAC	TGGGATTGCCAATGTAGTCG	55.0	50.0	58.1	58.0	394-838 bp	1.0	91.1
trnQ-UUG-ndhF_p4	TGGGCGAGACTTGACAAAAA	AATCGGGGTTACATGGATGC	45.0	50.0	58.0	58.0	487-931 bp	0.833333	84.8
trnQ-UUG-ndhF_p5	CATAGTGGGCGAGACTTGAC	AATCGGGGTTACATGGATGC	55.0	50.0	58.1	58.0	492-936 bp	0.833333	84.6