Hotspot

psbK-trnQ-UUG

Genome · 89576-90290 · 715 bp

Back to Drypetes

Pi 0.054

MarkerSeek score 77.20

Primer available yes

Label psbK-trnQ-UUG

Feature Metadata

label_name: psbK-trnQ-UUG
feature_type: igs
region: Genome
length_bp: 715
variable_sites: 73
pi: 0.054
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.77
markerseek_score: 77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-trnQ-UUG_p1	ACGCCCCGGATCATTAGATA	TGGGATTGCCAATGTAGTCG	50.0	50.0	58.1	58.0	1269-1695 bp	1.0	89.6
psbK-trnQ-UUG_p2	TACGCCCCGGATCATTAGAT	TGGGATTGCCAATGTAGTCG	50.0	50.0	58.1	58.0	1270-1696 bp	1.0	89.6
psbK-trnQ-UUG_p3	ACGCCCCGGATCATTAGATA	AATCGGGGTTACATGGATGC	50.0	50.0	58.1	58.0	1367-1793 bp	0.833333	82.6
psbK-trnQ-UUG_p4	TACGCCCCGGATCATTAGAT	AATCGGGGTTACATGGATGC	50.0	50.0	58.1	58.0	1368-1794 bp	0.833333	82.6
psbK-trnQ-UUG_p5	AGGCTTTTGTTTACCGAGGG	AATCGGGGTTACATGGATGC	50.0	50.0	58.1	58.0	1455-1891 bp	0.833333	82.1