Hotspot

psbZ-trnG-GCC

Genome · 79635-80344 · 710 bp

Back to Drypetes

Pi 0.0509
MarkerSeek score 81.70
Primer available yes
Label psbZ-trnG-GCC

Feature Metadata

label_name
psbZ-trnG-GCC
feature_type
igs
region
Genome
length_bp
710
variable_sites
77
pi
0.0509
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.89
markerseek_score
81.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GCC_p1 CCGGTACATCATTATGGATTGGA ACCATTAAACCATATCCGC 43.5 42.1 58.1 52.7 737-794 bp 1.0 52.0
psbZ-trnG-GCC_p2 TCCGGTACATCATTATGGATTGG ACCATTAAACCATATCCGC 43.5 42.1 58.1 52.7 738-795 bp 1.0 52.0
psbZ-trnG-GCC_p3 CCGGTACATCATTATGGATTGG ACCATTAAACCATATCCGC 45.5 42.1 56.7 52.7 737-794 bp 1.0 52.0
psbZ-trnG-GCC_p4 CGGTACATCATTATGGATTGGA ACCATTAAACCATATCCGC 40.9 42.1 55.7 52.7 736-793 bp 1.0 52.0
psbZ-trnG-GCC_p5 TCCGGTACATCATTATGGATTG ACCATTAAACCATATCCGC 40.9 42.1 55.7 52.7 738-795 bp 1.0 52.0