Hotspot

trnR-UCU-atpA

Genome · 51704-51851 · 148 bp

Back to Drypetes

Pi 0.0881

MarkerSeek score 77.60

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: Genome
length_bp: 148
variable_sites: 25
pi: 0.0881
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 77.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	GGTTCAAATCCTATTGGACGC	ACATTCACTGAAGAAGCGGA	47.6	45.0	57.3	57.2	226-325 bp	1.0	71.3
trnR-UCU-atpA_p2	GGTTCAAATCCTATTGGACGC	ACATTCACTGAAGAAGCGGAA	47.6	42.9	57.3	57.9	226-325 bp	1.0	69.0
trnR-UCU-atpA_p3	GGTTCAAATCCTATTGGACGC	AACATTCACTGAAGAAGCGGA	47.6	42.9	57.3	57.9	227-326 bp	1.0	68.9
trnR-UCU-atpA_p4	GGTTCAAATCCTATTGGACGC	CGGAAGCCCTTTTGAAAGAAG	47.6	47.6	57.3	57.7	210-309 bp	1.0	68.1
trnR-UCU-atpA_p5	GGTTCAAATCCTATTGGACGC	CGGAAGCCCTTTTGAAAGAA	47.6	45.0	57.3	56.5	210-309 bp	1.0	66.7