Hotspot

rbcL-trnV-UAC

Genome · 39873-40666 · 794 bp

Back to Drypetes

Pi 0.0927
MarkerSeek score 78.00
Primer available yes
Label rbcL-trnV-UAC

Feature Metadata

label_name
rbcL-trnV-UAC
feature_type
igs
region
Genome
length_bp
794
variable_sites
104
pi
0.0927
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.57
markerseek_score
78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-trnV-UAC_p1 TTTACACCAGCCTTGAACCC CCGAGAAAGTCTACGGTTCG 50.0 55.0 58.0 58.1 481-883 bp 1.0 91.1
rbcL-trnV-UAC_p2 AGCCTTGAACCCAACACTTG CCGAGAAAGTCTACGGTTCG 50.0 55.0 58.6 58.1 473-875 bp 1.0 86.4
rbcL-trnV-UAC_p3 CAGCCTTGAACCCAACACTT CCGAGAAAGTCTACGGTTCG 50.0 55.0 58.6 58.1 474-876 bp 1.0 86.4
rbcL-trnV-UAC_p4 CCTTGAACCCAACACTTGCT CCGAGAAAGTCTACGGTTCG 50.0 55.0 58.6 58.1 471-873 bp 1.0 86.4
rbcL-trnV-UAC_p5 TTTACACCAGCCTTGAACCC CGAGAAAGTCTACGGTTCGA 50.0 50.0 58.0 57.1 480-882 bp 1.0 84.8