Hotspot

accD-rbcL

Genome · 35470-38444 · 2975 bp

Back to Drypetes

Pi 0.241

MarkerSeek score 76.60

Primer available yes

Label accD-rbcL

Feature Metadata

label_name: accD-rbcL
feature_type: igs
region: Genome
length_bp: 2975
variable_sites: 1348
pi: 0.241
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 76.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-rbcL_p1	CGACGTTTCCTTTTGAACCG	TGCAGATACGGAGCTGGATA	50.0	50.0	58.0	58.0	494-549 bp	0.333333	55.8
accD-rbcL_p2	CGGTTCGACGTTTCCTTTTG	TGCAGATACGGAGCTGGATA	50.0	50.0	58.0	58.0	499-554 bp	0.333333	55.8
accD-rbcL_p3	TTACAAAGAACAGCGGGCTT	TGCAGATACGGAGCTGGATA	45.0	50.0	58.0	58.0	642-697 bp	0.333333	55.4
accD-rbcL_p4	GCTCAGCAGATCAAACTCGA	TGCAGATACGGAGCTGGATA	50.0	50.0	58.0	58.0	764 bp	0.166667	46.6
accD-rbcL_p5	GCTCAGCAGATCAAACTCGA	CTAAACGAGGGCATCCGAAA	50.0	50.0	58.0	58.0	639 bp	0.166667	46.5