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trnH-GUG-psbA

Genome · 99-535 · 437 bp

Back to Drypetes

Pi 0.0614

MarkerSeek score 81.40

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: Genome
length_bp: 437
variable_sites: 56
pi: 0.0614
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	TGACCACTTGGCTACATTCG	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.9	57.9	197-523 bp	1.0	90.7
trnH-GUG-psbA_p2	TGACCACTTGGCTACATTCG	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	200-526 bp	1.0	90.7
trnH-GUG-psbA_p3	TGACCACTTGGCTACATTCG	TAGACCTAGCTGCTGTCGAA	50.0	50.0	57.9	57.9	193-519 bp	1.0	90.3
trnH-GUG-psbA_p4	TGACCACTTGGCTACATTCG	CTAGACCTAGCTGCTGTCGA	50.0	55.0	57.9	58.3	194-520 bp	1.0	88.6
trnH-GUG-psbA_p5	TGACCACTTGGCTACATTCG	TCTAGACCTAGCTGCTGTCG	50.0	55.0	57.9	58.3	195-521 bp	1.0	88.6