primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	TGACCACTTGGCTACATTCG	CCTCTAGACCTAGCTGCTGT	20	20	50.0	55.0	57.9	57.9	0.0	13.4	0.159205	99	535	197-523	1.000000	90.7
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	TGACCACTTGGCTACATTCG	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.168035	99	535	200-526	1.000000	90.7
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	TGACCACTTGGCTACATTCG	TAGACCTAGCTGCTGTCGAA	20	20	50.0	50.0	57.9	57.9	0.0	13.4	0.217583	99	535	193-519	1.000000	90.3
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	TGACCACTTGGCTACATTCG	CTAGACCTAGCTGCTGTCGA	20	20	50.0	55.0	57.9	58.3	0.0	13.4	0.423702	99	535	194-520	1.000000	88.6
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	TGACCACTTGGCTACATTCG	TCTAGACCTAGCTGCTGTCG	20	20	50.0	55.0	57.9	58.3	0.0	13.4	0.423702	99	535	195-521	1.000000	88.6
trnK-UUU-trnQ-UUG_p1	trnK-UUU-trnQ-UUG	1	GGACGAATCCGCTTCTTCAT	GTTCGAAACCTTCCGTCCC	20	19	50.0	57.9	58.1	58.5	0.0	0.0	1.521950	4505	5335	791-983	1.000000	79.8
trnK-UUU-trnQ-UUG_p2	trnK-UUU-trnQ-UUG	2	CCGATGGTATGGACGAATCC	GTTCGAAACCTTCCGTCCC	20	19	55.0	57.9	57.9	58.5	0.0	0.0	1.543561	4505	5335	801-993	1.000000	79.7
trnK-UUU-trnQ-UUG_p3	trnK-UUU-trnQ-UUG	3	AAAGCCGAGTACTCTACCGT	GTTCGAAACCTTCCGTCCC	20	19	50.0	57.9	58.2	58.5	20.3	0.0	1.631695	4505	5335	740-932	1.000000	78.9
trnK-UUU-trnQ-UUG_p4	trnK-UUU-trnQ-UUG	4	AAGCCGAGTACTCTACCGTT	GTTCGAAACCTTCCGTCCC	20	19	50.0	57.9	58.2	58.5	20.3	0.0	1.631695	4505	5335	739-931	1.000000	78.9
trnK-UUU-trnQ-UUG_p5	trnK-UUU-trnQ-UUG	5	GTTAGCCGCACTTAAAAGCC	GTTCGAAACCTTCCGTCCC	20	19	50.0	57.9	57.7	58.5	0.0	0.0	1.721249	4505	5335	754-946	1.000000	78.2
trnQ-UUG-psbK_p1	trnQ-UUG-psbK	1	ATAGGTATGCTCTGGGACGG	AAGAGGACAGGGATTACGGG	20	20	55.0	55.0	58.4	58.5	0.0	0.0	0.869065	5408	6122	595-1015	1.000000	84.5
trnQ-UUG-psbK_p2	trnQ-UUG-psbK	2	ATAGGTATGCTCTGGGACGG	CAGGGATTACGGGCATAACA	20	20	55.0	50.0	58.4	57.4	0.0	0.0	0.997387	5408	6122	588-1008	1.000000	83.5
trnQ-UUG-psbK_p3	trnQ-UUG-psbK	3	ATAGGTATGCTCTGGGACGG	ACAGGGATTACGGGCATAAC	20	20	55.0	50.0	58.4	57.4	0.0	0.0	0.999213	5408	6122	589-1009	1.000000	83.5
trnQ-UUG-psbK_p4	trnQ-UUG-psbK	4	GACCAAAACCCGTTGCCTTA	AAGAGGACAGGGATTACGGG	20	20	50.0	55.0	58.7	58.5	0.0	0.0	1.182332	5408	6122	550-970	1.000000	82.3
trnQ-UUG-psbK_p5	trnQ-UUG-psbK	5	ATAGGTATGCTCTGGGACGG	TAGCAGCTTGCCAAACAAAG	20	20	55.0	45.0	58.4	57.2	0.0	0.0	1.165203	5408	6122	627-1047	1.000000	81.9
rpl14-rps8_p1	rpl14-rps8	1	ATTTTTGGTGCGATCCTCGA	AGCCATGTCAGCATTTCGTA	20	20	45.0	45.0	57.9	57.6	0.0	0.0	0.509533	12392	12601	338-377	1.000000	82.4
rpl14-rps8_p2	rpl14-rps8	2	ATTTTTGGTGCGATCCTCGA	TAGCCATGTCAGCATTTCGT	20	20	45.0	45.0	57.9	57.6	0.0	0.0	0.509533	12392	12601	339-378	1.000000	82.4
rpl14-rps8_p3	rpl14-rps8	3	TTTTTGGTGCGATCCTCGAG	AGCCATGTCAGCATTTCGTA	20	20	50.0	45.0	58.6	57.6	5.1	0.0	0.962932	12392	12601	337-376	1.000000	78.8
rpl14-rps8_p4	rpl14-rps8	4	TTTTTGGTGCGATCCTCGAG	TAGCCATGTCAGCATTTCGT	20	20	50.0	45.0	58.6	57.6	5.1	0.0	0.962932	12392	12601	338-377	1.000000	78.8
rpl14-rps8_p5	rpl14-rps8	5	ATTTTTGGTGCGATCCTCGA	AGCCATGTCAGCATTTCGTAT	20	21	45.0	42.9	57.9	58.1	0.0	0.0	1.184829	12392	12601	338-377	1.000000	77.0
accD_p1	accD	1	TTACGCCTGCATAAGAACCC	CAAAAGGAAACGTCGAACCG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.045966	34378	35469	2090-2296	0.666667	78.3
accD_p2	accD	2	CCATTGCAATTGCCGGAAAT	CAAAAGGAAACGTCGAACCG	20	20	45.0	50.0	58.0	58.0	26.9	0.0	0.040289	34378	35469	1913-2168	0.666667	77.8
accD_p3	accD	3	CCATTGCAATTGCCGGAAAT	TCGAGTTTGATCTGCTGAGC	20	20	45.0	50.0	58.0	58.0	26.9	0.0	0.028815	34378	35469	1684-1684	0.166667	46.4
accD_p4	accD	4	TTACGCCTGCATAAGAACCC	TCGAGTTTGATCTGCTGAGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.034492	34378	35469	1861-1861	0.166667	46.4
accD_p5	accD	5	TTGCCGGAAATACTAAGCCC	TCGAGTTTGATCTGCTGAGC	20	20	50.0	50.0	58.0	58.0	5.0	0.0	0.048814	34378	35469	1675-1675	0.166667	46.3
accD-rbcL_p1	accD-rbcL	1	CGACGTTTCCTTTTGAACCG	TGCAGATACGGAGCTGGATA	20	20	50.0	50.0	58.0	58.0	0.0	16.0	0.017909	36032	36151	494-549	0.333333	55.8
accD-rbcL_p2	accD-rbcL	2	CGGTTCGACGTTTCCTTTTG	TGCAGATACGGAGCTGGATA	20	20	50.0	50.0	58.0	58.0	0.0	16.0	0.017909	36032	36151	499-554	0.333333	55.8
accD-rbcL_p3	accD-rbcL	3	TTACAAAGAACAGCGGGCTT	TGCAGATACGGAGCTGGATA	20	20	45.0	50.0	58.0	58.0	0.0	16.0	0.028964	36032	36151	642-697	0.333333	55.4
accD-rbcL_p4	accD-rbcL	4	GCTCAGCAGATCAAACTCGA	TGCAGATACGGAGCTGGATA	20	20	50.0	50.0	58.0	58.0	0.0	16.0	0.006436	36032	36151	764-764	0.166667	46.6
accD-rbcL_p5	accD-rbcL	5	GCTCAGCAGATCAAACTCGA	CTAAACGAGGGCATCCGAAA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.020354	36032	36151	639-639	0.166667	46.5
rbcL-trnV-UAC_p1	rbcL-trnV-UAC	1	TTTACACCAGCCTTGAACCC	CCGAGAAAGTCTACGGTTCG	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.117826	39873	40666	481-883	1.000000	91.1
rbcL-trnV-UAC_p2	rbcL-trnV-UAC	2	AGCCTTGAACCCAACACTTG	CCGAGAAAGTCTACGGTTCG	20	20	50.0	55.0	58.6	58.1	0.0	0.0	0.704745	39873	40666	473-875	1.000000	86.4
rbcL-trnV-UAC_p3	rbcL-trnV-UAC	3	CAGCCTTGAACCCAACACTT	CCGAGAAAGTCTACGGTTCG	20	20	50.0	55.0	58.6	58.1	0.0	0.0	0.704745	39873	40666	474-876	1.000000	86.4
rbcL-trnV-UAC_p4	rbcL-trnV-UAC	4	CCTTGAACCCAACACTTGCT	CCGAGAAAGTCTACGGTTCG	20	20	50.0	55.0	58.6	58.1	0.0	0.0	0.704745	39873	40666	471-873	1.000000	86.4
rbcL-trnV-UAC_p5	rbcL-trnV-UAC	5	TTTACACCAGCCTTGAACCC	CGAGAAAGTCTACGGTTCGA	20	20	50.0	50.0	58.0	57.1	0.0	0.0	0.898807	39873	40666	480-882	1.000000	84.8
trnR-UCU-atpA_p1	trnR-UCU-atpA	1	GGTTCAAATCCTATTGGACGC	ACATTCACTGAAGAAGCGGA	21	20	47.6	45.0	57.3	57.2	0.0	0.0	2.532277	51704	51851	226-325	1.000000	71.3
trnR-UCU-atpA_p2	trnR-UCU-atpA	2	GGTTCAAATCCTATTGGACGC	ACATTCACTGAAGAAGCGGAA	21	21	47.6	42.9	57.3	57.9	0.0	0.0	2.818792	51704	51851	226-325	1.000000	69.0
trnR-UCU-atpA_p3	trnR-UCU-atpA	3	GGTTCAAATCCTATTGGACGC	AACATTCACTGAAGAAGCGGA	21	21	47.6	42.9	57.3	57.9	0.0	0.0	2.818792	51704	51851	227-326	1.000000	68.9
trnR-UCU-atpA_p4	trnR-UCU-atpA	4	GGTTCAAATCCTATTGGACGC	CGGAAGCCCTTTTGAAAGAAG	21	21	47.6	47.6	57.3	57.7	0.0	5.1	2.981600	51704	51851	210-309	1.000000	68.1
trnR-UCU-atpA_p5	trnR-UCU-atpA	5	GGTTCAAATCCTATTGGACGC	CGGAAGCCCTTTTGAAAGAA	21	20	47.6	45.0	57.3	56.5	0.0	5.1	3.157860	51704	51851	210-309	1.000000	66.7
psbZ-trnG-GCC_p1	psbZ-trnG-GCC	1	CCGGTACATCATTATGGATTGGA	ACCATTAAACCATATCCGC	23	19	43.5	42.1	58.1	52.7	0.0	0.0	9.350148	79635	80344	737-794	1.000000	52.0
psbZ-trnG-GCC_p2	psbZ-trnG-GCC	2	TCCGGTACATCATTATGGATTGG	ACCATTAAACCATATCCGC	23	19	43.5	42.1	58.1	52.7	0.0	0.0	9.350148	79635	80344	738-795	1.000000	52.0
psbZ-trnG-GCC_p3	psbZ-trnG-GCC	3	CCGGTACATCATTATGGATTGG	ACCATTAAACCATATCCGC	22	19	45.5	42.1	56.7	52.7	0.0	0.0	9.552926	79635	80344	737-794	1.000000	52.0
psbZ-trnG-GCC_p4	psbZ-trnG-GCC	4	CGGTACATCATTATGGATTGGA	ACCATTAAACCATATCCGC	22	19	40.9	42.1	55.7	52.7	0.0	0.0	10.505823	79635	80344	736-793	1.000000	52.0
psbZ-trnG-GCC_p5	psbZ-trnG-GCC	5	TCCGGTACATCATTATGGATTG	ACCATTAAACCATATCCGC	22	19	40.9	42.1	55.7	52.7	0.0	0.0	10.505823	79635	80344	738-795	1.000000	52.0
psbK-trnQ-UUG_p1	psbK-trnQ-UUG	1	ACGCCCCGGATCATTAGATA	TGGGATTGCCAATGTAGTCG	20	20	50.0	50.0	58.1	58.0	0.7	0.0	0.103936	89576	90290	1269-1695	1.000000	89.6
psbK-trnQ-UUG_p2	psbK-trnQ-UUG	2	TACGCCCCGGATCATTAGAT	TGGGATTGCCAATGTAGTCG	20	20	50.0	50.0	58.1	58.0	0.7	0.0	0.103936	89576	90290	1270-1696	1.000000	89.6
psbK-trnQ-UUG_p3	psbK-trnQ-UUG	3	ACGCCCCGGATCATTAGATA	AATCGGGGTTACATGGATGC	20	20	50.0	50.0	58.1	58.0	0.7	0.0	0.085722	89576	90290	1367-1793	0.833333	82.6
psbK-trnQ-UUG_p4	psbK-trnQ-UUG	4	TACGCCCCGGATCATTAGAT	AATCGGGGTTACATGGATGC	20	20	50.0	50.0	58.1	58.0	0.7	0.0	0.085722	89576	90290	1368-1794	0.833333	82.6
psbK-trnQ-UUG_p5	psbK-trnQ-UUG	5	AGGCTTTTGTTTACCGAGGG	AATCGGGGTTACATGGATGC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.113106	89576	90290	1455-1891	0.833333	82.1
trnQ-UUG-ndhF_p1	trnQ-UUG-ndhF	1	TGGGCGAGACTTGACAAAAA	TGGGATTGCCAATGTAGTCG	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.082519	90363	90461	389-833	1.000000	91.3
trnQ-UUG-ndhF_p2	trnQ-UUG-ndhF	2	TGGGCGAGACTTGACAAAAA	AAAATATGTGGGAGGCGGTC	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.098344	90363	90461	338-782	1.000000	91.2
trnQ-UUG-ndhF_p3	trnQ-UUG-ndhF	3	CATAGTGGGCGAGACTTGAC	TGGGATTGCCAATGTAGTCG	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.109063	90363	90461	394-838	1.000000	91.1
trnQ-UUG-ndhF_p4	trnQ-UUG-ndhF	4	TGGGCGAGACTTGACAAAAA	AATCGGGGTTACATGGATGC	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.064305	90363	90461	487-931	0.833333	84.8
trnQ-UUG-ndhF_p5	trnQ-UUG-ndhF	5	CATAGTGGGCGAGACTTGAC	AATCGGGGTTACATGGATGC	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.090849	90363	90461	492-936	0.833333	84.6
ndhD-psaC_p1	ndhD-psaC	1	CCGCGGATATCGGTAAAACT	AAACAACTCGAAGCATGGGT	20	20	50.0	45.0	57.9	58.0	24.1	0.0	0.163225	96535	96673	222-228	1.000000	85.1
ndhD-psaC_p2	ndhD-psaC	2	CCGCGGATATCGGTAAAACT	GGCATGAAACAACTCGAAGC	20	20	50.0	50.0	57.9	58.0	24.1	0.0	0.175378	96535	96673	228-234	1.000000	84.9
ndhD-psaC_p3	ndhD-psaC	3	CCGCGGATATCGGTAAAACT	AGAGGACTGTGTCGGTTGTA	20	20	50.0	50.0	57.9	58.0	24.1	0.0	0.153361	96535	96673	302-308	1.000000	83.5
ndhD-psaC_p4	ndhD-psaC	4	CCGCGGATATCGGTAAAACT	AAGAGATGTGAATCCGCCTG	20	20	50.0	50.0	57.9	57.7	24.1	0.0	0.466837	96535	96673	283-289	1.000000	81.3
ndhD-psaC_p5	ndhD-psaC	5	CCGCGGATATCGGTAAAACT	GAGATGTGAATCCGCCTGTC	20	20	50.0	55.0	57.9	58.4	24.1	0.0	0.568126	96535	96673	281-287	1.000000	80.6