Hotspot

ycf1

Genome · 107625-112760 · 5136 bp

Back to Dryopteris

Pi 0.0248

MarkerSeek score 55.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: Genome
length_bp: 5136
variable_sites: 246
pi: 0.0248
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CGCACGCAACTAGTAGAAGT	TCAGGCTGGTAAGGGGTTAA	50.0	50.0	58.0	58.0	257 bp	1.0	86.3
ycf1_p2	CGCACGCAACTAGTAGAAGT	GTAACTAGCCACGGCATCAA	50.0	50.0	58.0	58.0	932 bp	1.0	83.6
ycf1_p3	AGAACCAAGCAAGCCTATCG	TCAGGCTGGTAAGGGGTTAA	50.0	50.0	58.0	58.0	863 bp	0.75	74.5
ycf1_p4	AGAACCAAGCAAGCCTATCG	GTAACTAGCCACGGCATCAA	50.0	50.0	58.0	58.0	1538 bp	0.75	73.7
ycf1_p5	CGCACGCAACTAGTAGAAGT	GACATTTTCGTCCACTTGCG	50.0	50.0	58.0	58.0	616 bp	0.75	73.5