Hotspot

psaC

Genome · 118825-119070 · 246 bp

Back to Dryopteris

Pi 0.0102

MarkerSeek score 44.60

Primer available yes

Label psaC

Feature Metadata

label_name: psaC
feature_type: gene
region: Genome
length_bp: 246
variable_sites: 5
pi: 0.0102
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC_p1	GCTCCTTCTCTTCAGCGAAA	GGAACTGTGTGGGCCAATTA	50.0	50.0	57.9	58.1	737-747 bp	1.0	83.5
psaC_p2	GGCAGGCTTTCAAAAAGCAA	GGAACTGTGTGGGCCAATTA	45.0	50.0	58.0	58.1	532-535 bp	1.0	82.7
psaC_p3	GGCAGGCTTTCAAAAAGCAA	TGGAAAGTCCGTCGATACCT	45.0	50.0	58.0	58.2	835-838 bp	1.0	82.0
psaC_p4	GCTCCTTCTCTTCAGCGAAA	CCACCAGCCGTGTATAAGAC	50.0	55.0	57.9	58.1	1302-1306 bp	0.5	60.9
psaC_p5	GGCAGGCTTTCAAAAAGCAA	CCACCAGCCGTGTATAAGAC	45.0	55.0	58.0	58.1	1097-1098 bp	0.5	60.6