Hotspot

ndhF

Genome · 124767-126608 · 1842 bp

Back to Dryopteris

Pi 0.0283

MarkerSeek score 63.40

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: Genome
length_bp: 1842
variable_sites: 100
pi: 0.0283
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TGGATTTAATACGCCCGTGG	TCTGTATCGATCGGTCACCA	50.0	50.0	58.0	58.0	2456 bp	1.0	83.2
ndhF_p2	TGGATTTAATACGCCCGTGG	GTCGGGAGAAATGAACACGA	50.0	50.0	58.0	57.9	2295 bp	1.0	83.2
ndhF_p3	TGGATTTAATACGCCCGTGG	AGTCGGGAGAAATGAACACG	50.0	50.0	58.0	57.9	2296 bp	1.0	83.1
ndhF_p4	TGGATTTAATACGCCCGTGG	TCCCATTATAGGAGGAGGCG	50.0	55.0	58.0	58.1	2582-2589 bp	1.0	83.0
ndhF_p5	ACATGTGTCACGACTAGGGA	TCTGTATCGATCGGTCACCA	50.0	50.0	58.1	58.0	2100 bp	1.0	82.9