Hotspot

trnG-UCC-ycf12

Genome · 73570-73740 · 171 bp

Back to Dryopteris

Pi 0.0565
MarkerSeek score 77.80
Primer available yes
Label trnG-UCC-ycf12

Feature Metadata

label_name
trnG-UCC-ycf12
feature_type
igs
region
Genome
length_bp
171
variable_sites
19
pi
0.0565
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
77.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-ycf12_p1 ACGAGTCACACTTTTACCACT GCAGCTCGAAGGGGTAAC 42.9 61.1 57.2 57.8 224-229 bp 1.0 56.3
trnG-UCC-ycf12_p2 GAGACGAGTCACACTTTTACCA GCAGCTCGAAGGGGTAAC 45.5 61.1 58.0 57.8 227-232 bp 1.0 54.5
trnG-UCC-ycf12_p3 TGAGACGAGTCACACTTTTACC GCAGCTCGAAGGGGTAAC 45.5 61.1 58.0 57.8 228-233 bp 1.0 54.5
trnG-UCC-ycf12_p4 AGACGAGTCACACTTTTACCA GCAGCTCGAAGGGGTAAC 42.9 61.1 56.9 57.8 226-231 bp 1.0 54.0
trnG-UCC-ycf12_p5 GACGAGTCACACTTTTACCAC GCAGCTCGAAGGGGTAAC 47.6 61.1 56.9 57.8 225-230 bp 1.0 53.8