Hotspot

atpH-atpF

Genome · 69020-69371 · 352 bp

Back to Dryopteris

Pi 0.0442

MarkerSeek score 71.50

Primer available yes

Label atpH-atpF

Feature Metadata

label_name: atpH-atpF
feature_type: igs
region: Genome
length_bp: 352
variable_sites: 29
pi: 0.0442
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpF_p1	GTATTGCGAGACAGCCAGAA	TAAACCAATACTTGCGGCGA	50.0	45.0	58.0	57.9	551-567 bp	0.75	73.6
atpH-atpF_p2	CCGATCGGCGTAGGAAATAG	TAAACCAATACTTGCGGCGA	55.0	45.0	58.0	57.9	890-906 bp	0.75	72.5
atpH-atpF_p3	AAGAACCATTGCCAAGCTCA	TAAACCAATACTTGCGGCGA	45.0	45.0	58.0	57.9	1155-1171 bp	0.75	72.1
atpH-atpF_p4	AGAACCATTGCCAAGCTCAA	TAAACCAATACTTGCGGCGA	45.0	45.0	58.0	57.9	1154-1170 bp	0.75	72.1
atpH-atpF_p5	AGAACCATTGCCAAGCTCAA	ACTCGGATATACGACTCGCA	45.0	50.0	58.0	58.1	1254-1270 bp	0.75	71.7