Hotspot

petN-psbM

Genome · 53061-54263 · 1203 bp

Back to Dryopteris

Pi 0.0353

MarkerSeek score 71.20

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: Genome
length_bp: 1203
variable_sites: 81
pi: 0.0353
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	CTTGCTACCGAACAATCCGA	CGTTTCTAGCTACCGCACTT	50.0	50.0	58.0	58.0	1977-1997 bp	1.0	85.6
petN-psbM_p2	AAACGCTAGATGTCGGTGAC	CGTTTCTAGCTACCGCACTT	50.0	50.0	58.0	58.0	2020-2040 bp	1.0	85.5
petN-psbM_p3	CTTGCTACCGAACAATCCGA	GGGCCCGATTACCTTTTAGG	50.0	55.0	58.0	58.0	2436-2456 bp	1.0	85.1
petN-psbM_p4	CTTGCTACCGAACAATCCGA	GGGGCCCGATTACCTTTTAG	50.0	55.0	58.0	58.0	2437-2457 bp	1.0	85.1
petN-psbM_p5	AAACGCTAGATGTCGGTGAC	GGGCCCGATTACCTTTTAGG	50.0	55.0	58.0	58.0	2479-2499 bp	1.0	85.1