Hotspot

trnG-UCC-trnC-GCA

Genome · 51938-52434 · 497 bp

Back to Dryopteris

Pi 0.0369

MarkerSeek score 73.90

Primer available yes

Label trnG-UCC-trnC-GCA

Feature Metadata

label_name: trnG-UCC-trnC-GCA
feature_type: igs
region: Genome
length_bp: 497
variable_sites: 36
pi: 0.0369
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnC-GCA_p1	GTTCGATTCCCGCTATCCG	GATTTGCAGTCCTCCGTCTT	57.9	50.0	58.2	57.9	554-557 bp	1.0	75.3
trnG-UCC-trnC-GCA_p2	GTTCGATTCCCGCTATCCG	TTACCGCTTGACCATATCGC	57.9	50.0	58.2	58.1	536-539 bp	1.0	75.2
trnG-UCC-trnC-GCA_p3	GGGTTCGATTCCCGCTATC	GATTTGCAGTCCTCCGTCTT	57.9	50.0	57.8	57.9	556-559 bp	1.0	75.1
trnG-UCC-trnC-GCA_p4	GGTTCGATTCCCGCTATCC	GATTTGCAGTCCTCCGTCTT	57.9	50.0	57.8	57.9	555-558 bp	1.0	75.1
trnG-UCC-trnC-GCA_p5	GGTTCGATTCCCGCTATCC	TTACCGCTTGACCATATCGC	57.9	50.0	57.8	58.1	537-540 bp	1.0	75.0