Hotspot

psbJ-petA

Genome · 21938-22667 · 730 bp

Back to Dryopteris

Pi 0.0495

MarkerSeek score 80.00

Primer available yes

Label psbJ-petA

Feature Metadata

label_name: psbJ-petA
feature_type: igs
region: Genome
length_bp: 730
variable_sites: 69
pi: 0.0495
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbJ-petA_p1	AGGTCCGTCAGGCATATTCT	GCAGTTGGCAGAAATGAATT	50.0	40.0	58.2	55.1	816-826 bp	1.0	62.1
psbJ-petA_p2	TACGGAGCTTACTCAGGGTT	GCAGTTGGCAGAAATGAATT	50.0	40.0	57.8	55.1	794-804 bp	1.0	62.1
psbJ-petA_p3	TTACGGAGCTTACTCAGGGT	GCAGTTGGCAGAAATGAATT	50.0	40.0	57.8	55.1	795-805 bp	1.0	62.1
psbJ-petA_p4	AGGTCCGTCAGGCATATTCT	GCAGTTGGCAGAAATGAATTTC	50.0	40.9	58.2	57.1	816-826 bp	1.0	61.9
psbJ-petA_p5	TACGGAGCTTACTCAGGGTT	GCAGTTGGCAGAAATGAATTTC	50.0	40.9	57.8	57.1	794-804 bp	1.0	61.9