Hotspot

rpl23-rpl2

Genome · 553-620 · 68 bp

Back to Dryopteris

Pi 0.0907

MarkerSeek score 73.10

Primer available yes

Label rpl23-rpl2

Feature Metadata

label_name: rpl23-rpl2
feature_type: igs
region: Genome
length_bp: 68
variable_sites: 12
pi: 0.0907
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl23-rpl2_p1	TAATTGGCGAATTCACGGGT	TTCCGTTGTTTCCCCGAATT	45.0	45.0	57.9	58.0	617 bp	1.0	84.5
rpl23-rpl2_p2	TAATTGGCGAATTCACGGGT	TCAGTTTGTCTCGCCGAAAA	45.0	45.0	57.9	58.1	756 bp	1.0	83.6
rpl23-rpl2_p3	TAATTGGCGAATTCACGGGT	CTTCGGTATACGCATAGGGC	45.0	55.0	57.9	58.0	1204 bp	1.0	83.3
rpl23-rpl2_p4	TAATTGGCGAATTCACGGGT	GCCTTCGGTATACGCATAGG	45.0	55.0	57.9	58.0	1206 bp	1.0	83.3
rpl23-rpl2_p5	TAATTGGCGAATTCACGGGT	CCCGACTGCCAAATCGAATA	45.0	50.0	57.9	58.1	1247 bp	0.25	48.7