Hotspot

ycf1

SSC · 124094-129838 · 5745 bp

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Pi 0.0022

MarkerSeek score 47.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5745
variable_sites: 19
pi: 0.0022
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCCCATTCTCCAATGCTGAA	ACCATTGGTATTTCATTGGTTCA	45.0	34.8	56.8	56.7	262 bp	1.0	40.6
ycf1_p2	TTCCCATTCTCCAATGCTGA	ACCATTGGTATTTCATTGGTTCA	45.0	34.8	56.8	56.7	263 bp	1.0	40.6
ycf1_p3	TTCCCATTCTCCAATGCTGAA	ACCATTGGTATTTCATTGGTTCA	42.9	34.8	57.5	56.7	263 bp	1.0	40.6
ycf1_p4	TCCCATTCTCCAATGCTGAA	TCAAACCATTGGTATTTCATTGGT	45.0	33.3	56.8	57.3	266 bp	1.0	40.6
ycf1_p5	TTCCCATTCTCCAATGCTGA	TCAAACCATTGGTATTTCATTGGT	45.0	33.3	56.8	57.3	267 bp	1.0	40.6