Hotspot

rpl14

LSC · 80981-81349 · 369 bp

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Pi 0.00542

MarkerSeek score 56.30

Primer available yes

Label rpl14

Feature Metadata

label_name: rpl14
feature_type: gene
region: LSC
length_bp: 369
variable_sites: 3
pi: 0.00542
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14_p1	AAACTCTATCGCGGCATTCC	TCACCCTTTACATTGAAAAAGACA	50.0	33.3	58.4	57.0	433 bp	1.0	40.6
rpl14_p2	AAACTCTATCGCGGCATTCC	CACCCTTTACATTGAAAAAGACA	50.0	34.8	58.4	55.6	432 bp	1.0	40.6
rpl14_p3	AAACTCTATCGCGGCATTCC	TCACCCTTTACATTGAAAAAGACAA	50.0	32.0	58.4	57.6	433 bp	1.0	40.6
rpl14_p4	AAACTCTATCGCGGCATTCC	TTCACCCTTTACATTGAAAAAGACA	50.0	32.0	58.4	57.6	434 bp	1.0	40.6
rpl14_p5	AAACTCTATCGCGGCATTCC	AGGTTTCTTGTTTTGTTTTGAACA	50.0	29.2	58.4	56.6	476 bp	1.0	40.5