Hotspot

clpP

LSC · 70068-72170 · 2103 bp

Back to Drymocallis

Pi 0.00254
MarkerSeek score 59.30
Primer available yes
Label clpP

Feature Metadata

label_name
clpP
feature_type
gene
region
LSC
length_bp
2103
variable_sites
8
pi
0.00254
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
clpP_p1 TCACGAATTGATATGAAATCCTTGT AGGCCACTAAACCCATTGTT 32.0 45.0 57.1 57.3 2208-2213 bp 1.0 40.4
clpP_p2 CACGAATTGATATGAAATCCTTGT AGGCCACTAAACCCATTGTT 33.3 45.0 55.8 57.3 2207-2212 bp 1.0 40.4
clpP_p3 TCACGAATTGATATGAAATCCTTGTT AGGCCACTAAACCCATTGTT 30.8 45.0 57.7 57.3 2208-2213 bp 1.0 40.4
clpP_p4 TCACGAATTGATATGAAATCCTTG AGGCCACTAAACCCATTGTT 33.3 45.0 55.6 57.3 2208-2213 bp 1.0 40.4
clpP_p5 ATCACGAATTGATATGAAATCCTTGT AGGCCACTAAACCCATTGTT 30.8 45.0 57.5 57.3 2209-2214 bp 1.0 40.4