Hotspot

cemA

LSC · 61026-61715 · 690 bp

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Pi 0.000966

MarkerSeek score 33.50

Primer available yes

Label cemA

Feature Metadata

label_name: cemA
feature_type: gene
region: LSC
length_bp: 690
variable_sites: 1
pi: 0.000966
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 33.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cemA_p1	CGCATGGGGTGAGTTCATTA	TTGGGCCGTTCGATAATTTT	50.0	40.0	58.0	56.0	757 bp	1.0	63.9
cemA_p2	CGCATGGGGTGAGTTCATTA	AATTGGGCCGTTCGATAATT	50.0	40.0	58.0	55.8	759 bp	1.0	62.0
cemA_p3	CGCATGGGGTGAGTTCATTA	ATTGGGCCGTTCGATAATTT	50.0	40.0	58.0	55.8	758 bp	1.0	62.0
cemA_p4	CGCATGGGGTGAGTTCATTA	AATTGGGCCGTTCGATAATTT	50.0	38.1	58.0	56.6	759 bp	1.0	60.3
cemA_p5	CGCATGGGGTGAGTTCATTA	ATTGGGCCGTTCGATAATTTT	50.0	38.1	58.0	56.6	758 bp	1.0	60.3