Hotspot

petA-psbJ

LSC · 62887-63852 · 966 bp

Back to Drymocallis

Pi 0.00483

MarkerSeek score 61.20

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 966
variable_sites: 7
pi: 0.00483
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	AAGGTTCAGTTGGCCGAAAT	CCGGATTAGGTTCATCTCTGT	45.0	47.6	58.0	56.9	1018-1020 bp	1.0	63.8
petA-psbJ_p2	TTGAGAAGGTTCAGTTGGCC	CCGGATTAGGTTCATCTCTGT	50.0	47.6	58.0	56.9	1023-1025 bp	1.0	63.7
petA-psbJ_p3	ATCCATTACGTGTCCAAGGC	CCGGATTAGGTTCATCTCTGT	50.0	47.6	58.0	56.9	1107-1109 bp	1.0	63.5
petA-psbJ_p4	CATTACGTGTCCAAGGCCTT	CCGGATTAGGTTCATCTCTGT	50.0	47.6	58.2	56.9	1104-1106 bp	1.0	62.4
petA-psbJ_p5	AAGGTTCAGTTGGCCGAAAT	TCCGGATTAGGTTCATCTCTGT	45.0	45.5	58.0	58.4	1019-1021 bp	1.0	61.8