Hotspot

trnE-UUC-trnT-GGU

LSC · 30912-31440 · 529 bp

Back to Drymocallis

Pi 0.00252

MarkerSeek score 58.90

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 529
variable_sites: 2
pi: 0.00252
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	AGAGAGATGTCCTGGACCAC	GAACCGATGACTTACGCCTT	55.0	50.0	58.1	58.0	618 bp	1.0	79.1
trnE-UUC-trnT-GGU_p2	GAGAGATGTCCTGGACCACT	GAACCGATGACTTACGCCTT	55.0	50.0	58.1	58.0	617 bp	1.0	79.1
trnE-UUC-trnT-GGU_p3	AGAGAGATGTCCTGGACCAC	CCATGGCGTTACTCTACCAC	55.0	55.0	58.1	58.1	597 bp	1.0	78.6
trnE-UUC-trnT-GGU_p4	GAGAGATGTCCTGGACCACT	CCATGGCGTTACTCTACCAC	55.0	55.0	58.1	58.1	596 bp	1.0	78.6
trnE-UUC-trnT-GGU_p5	GAGAGATGTCCTGGACCACT	TGGCGTTACTCTACCACTGA	55.0	50.0	58.1	58.1	593 bp	1.0	78.5