Hotspot

rpoB-trnC-GCA

LSC · 26120-27309 · 1190 bp

Back to Drymocallis

Pi 0.00336

MarkerSeek score 54.00

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1190
variable_sites: 6
pi: 0.00336
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	TATTGCCTCATTTCCACCCC	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.5	58.1	1278-1280 bp	1.0	76.2
rpoB-trnC-GCA_p2	TATTGCCTCATTTCCACCCC	AAAAGGATTTGCAGTCCCCC	50.0	50.0	57.5	58.4	1260-1262 bp	1.0	74.0
rpoB-trnC-GCA_p3	CATTTCCACCCCCAAGCAT	CCGGATTTGAACTGGGGAAA	52.6	50.0	58.0	58.1	1270-1272 bp	1.0	71.8
rpoB-trnC-GCA_p4	ATATTGCCTCATTTCCACCCC	CCGGATTTGAACTGGGGAAA	47.6	50.0	58.0	58.1	1279-1281 bp	1.0	71.5
rpoB-trnC-GCA_p5	GTTCCTCTGTTAAGCCCTGA	CCGGATTTGAACTGGGGAAA	50.0	50.0	56.8	58.1	1354-1356 bp	1.0	70.6