Hotspot

rps16-trnQ-UUG

LSC · 6160-7066 · 907 bp

Back to Drymocallis

Pi 0.00226
MarkerSeek score 61.20
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
907
variable_sites
3
pi
0.00226
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.98
markerseek_score
61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 CAGCGGATCATGTCCTTCAA GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 953-998 bp 1.0 83.1
rps16-trnQ-UUG_p2 GCGGATCATGTCCTTCAAGT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 951-996 bp 1.0 83.1
rps16-trnQ-UUG_p3 GATGTAAGACTCCACAGCGG GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.1 58.1 967-1012 bp 1.0 82.7
rps16-trnQ-UUG_p4 GGATGTAAGACTCCACAGCG GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.1 58.1 968-1013 bp 1.0 82.7
rps16-trnQ-UUG_p5 TAAGACTCCACAGCGGATCA GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 963-1008 bp 1.0 82.1