Hotspot

ycf1

SSC · 121577-127150 · 5574 bp

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Pi 0.0716

MarkerSeek score 70.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5574
variable_sites: 391
pi: 0.0716
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGGACGACGAATCCGAAAAA	AACTTCCTTTTGGTTCCCCC	45.0	50.0	57.8	57.9	366 bp	0.5	58.0
ycf1_p2	TTGGACGACGAATCCGAAAA	AACTTCCTTTTGGTTCCCCC	45.0	50.0	57.8	57.9	367 bp	0.5	58.0
ycf1_p3	TTTGGACGACGAATCCGAAA	AACTTCCTTTTGGTTCCCCC	45.0	50.0	57.8	57.9	368 bp	0.5	58.0
ycf1_p4	TGGACGACGAATCCGAAAAA	CTTCCCGAGTGGTCTAAGGA	45.0	55.0	57.8	58.2	628 bp	0.5	57.6
ycf1_p5	TTGGACGACGAATCCGAAAA	CTTCCCGAGTGGTCTAAGGA	45.0	55.0	57.8	58.2	629 bp	0.5	57.6